2018-02-10T12:44:28.000Z2025-05-14 23:35:15

locus

BSU08090

BSU_08090

geneLength

1374

1377

outlinks

bsu

BSU08090

BSU_08090

Gene

Coordinates

882,266 → 883,642

882,266 883,642

The protein

Catalyzed reaction/ biological activity

Protein N(6)-(dihydrolipoyl)lysine + NAD⁺ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

Protein N⁶-(dihydrolipoyl)lysine + NAD⁺ --> protein N⁶-(lipoyl)lysine + NADH (according to UniProt)

The protein

Protein family

class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

class-I pyridine nucleotide-disulfide oxidoreductase family (with [[protein|PdhD]] and [[protein|LpdV]], according to UniProt)

The protein

Paralogous protein(s)

[[protein|LpdV]], [[protein|PdhD]]

[[this]]

The protein

[SW|Localization]

cytoplasm (according to Swiss-Prot)

cytoplasm (according to UniProt)

Biological materials

Mutant

MGNA-C351 (acoL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2349 NBRP B. subtilis, Japan]

BKE08090 (Δ[[gene|acoL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC

BKK08090 (Δ[[gene|acoL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC

MGNA-C351 (acoL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2349 NBRP B. subtilis, Japan]

BKE08090 ([[gene|acoL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC

BKK08090 ([[gene|acoL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC

Labs working on this gene/protein

[SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

The protein

[SW|Cofactors]

FAD (according to UniProt)

The protein

Structure

[PDB|2EQ7] (from Thermus thermophilus, 40% identity)

Expression and Regulation

additional information

the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

labs